Plant DNA Barcoding at the Michigan Community College Biologist's Conference

This workshop is part of the Michigan Community College Biologists Annual Conference. Workshop participants will carry out an entry-level experiment on plant identification using the rbcL barcode—from rapid DNA extraction to PCR and electrophoresis, sequencing library preparation, nanopore sequencing, read quality control and assembly, BLAST, multiple sequence alignment, and tree building. Bring a fresh leaf from a plant or two that you would like to barcode.
 
The workshop will combine three elements that form one of the most widely-used infrastructures to support independent, distributed, and course-based research. DNA barcoding is the “do everything lab” that merges field work, biochemistry, bioinformatics, genetics, ecology, and systematics. Nanopore sequencing, which analyzes individual DNA molecules in real time, is the “next big thing” in biology education. DNA Subway 2.0 is the only research-grade bioinformatics platform that is easy to use and runs smoothly on desktop computers, tablets, and cell phones. 
 
The DNA Learning Center (DNALC) and Oxford Nanopore Technologies are working together to adapt nanopore sequencing for use in education—including improved chemistry, workflows, experiments, and packaging/pricing attractive to biology educators. At less than $10 per sample, nanopore sequencing is a speedy and cost-effective alternative to commercial sequencing. Nanopore opens the “black box,” exposing students to every step of DNA sequencing. 
 
This workshop is funded by InnovATEBIO, the NSF National Biotechnology Education Center, which focuses on workforce development at community colleges. All participants will receive a $150 stipend.