ATE Project Talks: NISTCHO Cells for Teaching Biomanufacturing

ATE Project: 
  • Antibody Engineers
  • Filed under: 
    Presentation: 

    Description: NISTCHO cells are a newly released recombinant cell line that produce cNISTmAb, an industry relevant, open access monoclonal antibody to RSV. The NISTCHO cell line was developed by the National Institute of Standards and Technology (NIST) and MilliporeSigma and is available as a research grade test material (RGTM 10197). A lab-based curriculum has been developed to facilitate the use of the NIST CHO cell line in biomanufacturing courses and workforce development programs. Standard operating procedures, batch records and corresponding data will be shared for small scale NIST CHO growth and mAb production. Outcomes of undergraduate research projects on cryopreservation and stability studies will be discussed.

    You can collaborate with other faculty in this summer's Antibody Engineering Hackathon to help develop protocols for using NISTCHO cells in teaching and research projects.  Visit Antibody-Engineers.org to sign up for the mailing list and we will notify you when it's time to apply for the 2024 Hackathon.

    Speakers:  Margaret Bryans, Montgomery County Community College, PA and Lori Kelman, Montgomery College Germantown, MD

    Funding: This work was performed under financial assistance award 70NANB21H085 from U.S. Department of Commerce, National Institute of Standards and Technology and with funding from the National Science Foundation ATE program, Developing Classroom-based Undergraduate Research Experiences in Antibody Bioengineering NSF-ATE DUE 2055036.

    Links from the talk:

    1. Where to get NISTCHO cells This is the research grade material.

    2.  Protocol for using NISTCHO cells

    3. Protocols for working with antibodies - these are based on an earlier cell line but are in the process of being updated.

    4.  How to get on the mailing list for the 2024 Antibody Engineering Hackathon, Augst 5th-8th. We will start accepting applications April 1st, 2024.  Applicants must be a college faculty member, college students, or working in industry and be based in the US.