Protein chromatography

How can you teach strategies for protein purification without a lab?

Protein Purification is an award-winning program which has been widely used in schools, colleges and universities since 1983. It guides students (and experts!) through some of the more commonly-used protein separation techniques and lets them experiment. Students can begin with a simple mixture of proteins and follow a tutorial to see how these behave during gel filtration and ion-exchange chromatography. Afterwards, students can go on to design and test full purification protocols using more complex mixtures of proteins. The behavior of proteins in the... Read more

Bioscience Technology at Portland Community College is getting slippery! As our class forges ahead in the fun filled world of Bioseparations we tackled chromatography and protein gels (protein electrophoresis) this week! We learned how to piece together a gel set-up which is like negotiating a particularly tricky set of Legos. We also made our own resolving and stacking gels! Gels are fun!

There's a lot going on in running gels. Sure, I've seen a picture of a gel in textbooks. Our class even talked about them a bit in Current Topics last term, but I wasn't... Read more