Mock ELISA - A Teaching Tool

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Presenters:  Bridgette Kirkpatrick (Collin College)  and Kevin Simpson (Delgado Community College)

This workshop will be a hands-on activity, which includes a novel approach to introducing quantitative data analysis in an engaging contextual setting. Using a previously prepared ELISA based format, the class will be able to complete “disease” (or other) diagnostics and analysis in a BSL-1 environment with no specialized chemicals or equipment needed. The activity is designed to introduce students (secondary and college) to basic statistical analysis and the importance of proper curve generation and quality control. Examples of variability in standard curves and out of range control and patient samples will be included. This will be followed by discussion of examples of how ELISAs are used in the biotech industry, the consequences of improper analysis and the importance of data integrity.