My lab partner, Allison, and I have been assured that troubleshooting is a desired skill and that we will be grateful for our current Proteins woes later in our careers. And I'm proud to say that despite repeated setbacks, we've put our big-girl lab coats on, looked that ?-galactosidase in its ornery eye, and paraphrased the words of the incomparable "Rowdy" Roddy Piper in the best B movie ever, They Live:
"We have come here to chew bubblegum and purify proteins... and we're all outta bubblegum."
A run-down of the problems we've encountered and the ferreted out causes:... Read more